We start with n= 76. In these analyses, I used a stringent pipeline, censoring and interpolating over vols with > FD of 0.5 mm or DVARS > 1.75. Those are excluded from the timeseries used to calculate the networks used here. I excluded anyone with > 50% frames censored (following Yeo, 2011) or mean motion > 1 mm, or max motion over 10 mm, leaving us with 71 participants, 26 of which have a second run.
The dataframe below shows all runs for subjects who meet this criteria. At the moment, I’m just examining the first run, not averaging in those who have a second run.
| No | Variable | Stats / Values | Freqs (% of Valid) | Graph | Valid | Missing |
|---|---|---|---|---|---|---|
| 1 | age_scan [numeric] | mean (sd) : 6.98 (1.3) min < med < max : 4.11 < 7.04 < 10.59 IQR (CV) : 1.92 (0.19) | 69 distinct values | 93 (100%) | 0 (0%) | |
| 2 | run [factor] | 1. run-01 2. run-02 3. run-03 | 66 (71.0%) 26 (28.0%) 1 (1.1%) | 93 (100%) | 0 (0%) | |
| 3 | pctSpikesFD [numeric] | mean (sd) : 0.09 (0.1) min < med < max : 0 < 0.06 < 0.36 IQR (CV) : 0.11 (1.05) | 69 distinct values | 93 (100%) | 0 (0%) | |
| 4 | relMeanRMSMotion [numeric] | mean (sd) : 0.26 (0.16) min < med < max : 0.04 < 0.22 < 0.78 IQR (CV) : 0.18 (0.6) | 93 distinct values | 93 (100%) | 0 (0%) | |
| 5 | nSpikesDV [integer] | mean (sd) : 10.95 (9.14) min < med < max : 0 < 9 < 35 IQR (CV) : 15 (0.84) | 30 distinct values | 93 (100%) | 0 (0%) | |
| 6 | relMaxRMSMotion [numeric] | mean (sd) : 2.65 (2.3) min < med < max : 0.13 < 1.97 < 9.64 IQR (CV) : 3.07 (0.87) | 93 distinct values | 93 (100%) | 0 (0%) |
Generated by summarytools 0.8.7 (R version 3.4.3)
2019-08-04
## Adding missing grouping variables: `ID`
| No | Variable | Stats / Values | Freqs (% of Valid) | Graph | Valid | Missing |
|---|---|---|---|---|---|---|
| 1 | ID [factor] | 1. sub-CBPD0002 2. sub-CBPD0015 3. sub-CBPD0018 4. sub-CBPD0020 5. sub-CBPD0023 6. sub-CBPD0025 7. sub-CBPD0035 8. sub-CBPD0037 9. sub-CBPD0038 10. sub-CBPD0040 [ 66 others ] | 1 (1.4%) 0 (0.0%) 0 (0.0%) 1 (1.4%) 1 (1.4%) 1 (1.4%) 1 (1.4%) 1 (1.4%) 1 (1.4%) 1 (1.4%) 63 (88.8%) | 71 (100%) | 0 (0%) | |
| 2 | race2 [factor] | 1. White 2. Black 3. Other | 18 (26.5%) 41 (60.3%) 9 (13.2%) | 68 (95.77%) | 3 (4.23%) | |
| 3 | ethnicity [factor] | 1. Not Hispanic or Latino 2. Hispanic or Latino | 61 (85.9%) 10 (14.1%) | 71 (100%) | 0 (0%) | |
| 4 | has_diagnoses [integer] | mean (sd) : 0.03 (0.17) min < med < max : 0 < 0 < 1 IQR (CV) : 0 (5.92) | 0 : 69 (97.2%) 1 : 2 (2.8%) | 71 (100%) | 0 (0%) | |
| 5 | parent1_edu [integer] | mean (sd) : 14.91 (2.7) min < med < max : 12 < 14 < 20 IQR (CV) : 6 (0.18) | 12 : 26 (37.1%) 14 : 10 (14.3%) 16 : 14 (20.0%) 18 : 16 (22.9%) 20 : 4 (5.7%) | 70 (98.59%) | 1 (1.41%) | |
| 6 | parent2_edu [integer] | mean (sd) : 13.46 (4.36) min < med < max : 0 < 12 < 20 IQR (CV) : 4 (0.32) | 0 : 3 (5.1%) 10 : 4 (6.8%) 12 : 27 (45.8%) 14 : 4 (6.8%) 16 : 8 (13.6%) 18 : 7 (11.9%) 20 : 6 (10.2%) | 59 (83.1%) | 12 (16.9%) | |
| 7 | income_median [integer] | mean (sd) : 61963.24 (55210.88) min < med < max : 2500 < 42500 < 2e+05 IQR (CV) : 67000 (0.89) | 11 distinct values | 68 (95.77%) | 3 (4.23%) | |
| 8 | monthslive_iflostincome [integer] | mean (sd) : 2.7 (1.46) min < med < max : 1 < 3 < 5 IQR (CV) : 3 (0.54) | 1 : 20 (29.8%) 2 : 11 (16.4%) 3 : 17 (25.4%) 4 : 7 (10.4%) 5 : 12 (17.9%) | 67 (94.37%) | 4 (5.63%) | |
| 9 | childaces_sum_ignorenan [integer] | mean (sd) : 1.14 (1.38) min < med < max : 0 < 1 < 6 IQR (CV) : 2 (1.21) | 0 : 32 (45.1%) 1 : 16 (22.5%) 2 : 11 (15.5%) 3 : 8 (11.3%) 4 : 2 (2.8%) 5 : 1 (1.4%) 6 : 1 (1.4%) | 71 (100%) | 0 (0%) |
Generated by summarytools 0.8.7 (R version 3.4.3)
2019-08-04
WPPSI and WISC scores are not totally finalized, so decided not to look at cognition for the moment, unless environmental effects are not interesting. For the future.
We find that overall, all measures of network segregation significantly increase with age in our dataset, with remarkable consistency. We examined within-network connectivity, between network connectivity, system segregation (as calculated in Chan et al. 2018), the modularity quality index, and the participation coefficient (summed across negative and positive weights).
We controlled for sex, mean framewise displacement, percent of FD spikes in the data, the number of volumes a participant had, and the average weight of the functional network.
Interestingly, as a side note, the number of communities detected using modularity maximization on this data decreases slightly with age (range in k is 2, 5), but that looks to just be that we don’t have many samples above 8 years old.
We also used restricted least ratio tests to test for the presence of non-linearity in our data. As you can see in the plots below, which use GAMs, most measures are linear and look no different from the linear models above.
Tests of non-linearity confirmed that no non-linear effects are present. The wiggly participation coefficient line is just over-fitting.
We fit the same model, controlling for sex, mean framewise displacement, percent of FD spikes in the data, the number of volumes a participant had, and the average weight of the functional network, to between- and within-system connectivity for each of the Yeo 7 systems.
We find the strongest effects are in the default mode system, between default and attentional networks.
None of these showed significantly non-linear effects, either.
Effects on default mode connectivity survive FDR correction across the tests conducted, with DMN-VAN p_fdr=0.0075545 and DMN-DAN p_fdr= 0.0085395. Vis-DAN p_fdr= 0.071222.
In summary, there are no main effects of SES, race, or ACES sum on any of the measures of network segregation.
Using the same models as above, age_scan + male + fd_mean + avgweight + pctSpikesFD + size_t, we see no significant associations between any of the measures of network segregation and either parent 1 education, family income (median of bin), or an SES composite of the two. Race is also not significantly associated with any of the measures of network segregation. I also looked at the sum of child ACES, which doesn’t show any significant associations in the model above.
What about the neighborhood questionnaire? That is not in the data that I have.
No significant interactions between any of the environmental variables above and age. However, age x income is marginally predictive of within- and between-network connectivity.
p=0.0829168 for within-network connectivity and p=0.0829168 for between-network connectivity. However, this does not seem consistent across different SES metrics (education vs. income, etc.)
I decided to look specifically at the networks that show strong age effects in our age range, that is, visual to dorsal attention and DMN to attentional networks, with the idea that previously the systems that showed stronger age effects also showed environmental interactions.
Visual to DAN
DMN to DAN
DMN to VAN
Visual to DAN
DMN to DAN
DMN to VAN
There are also sometimes outliers on some of the measures that flatten out age or environment trends, I have been looking into using robust regression to examine these trends instead, but did not go through all the trouble to bootstrap p-values for this time around. However, I think it’s possible that robust regression might be a more defensible choice.
We used Schaefer200 as the replication parcellation, since it has nice correspondence to Schaefer400 and the Yeo7 systems.
All previous findings significant increases in network segregation with age hold in our replication parcellation! The only finding that is inconsistent is the number of communities detected using modularity maximization decreasing with age, which was likely spurious anyways.
As a reminder, we controlled for sex, mean framewise displacement, percent of FD spikes in the data, the number of volumes a participant had, and the average weight of the functional network.
Also, no non-linear effects of age.
Again, we find the strongest effects in Vis-DAN, DMN-DAN, and DMN-VAN, which are the only effects that pass fdr correction.
All three effects survive FDR correction across the tests conducted, with DMN-VAN p_fdr=0.0027812, DMN-DAN p_fdr= 0.0366607, Vis-DAN p_fdr= 0.036617.
Again, the effects show above replicate.
Visual to DAN
DMN to DAN
DMN to DAN
Visual to DAN
DMN to DAN
DMN to VAN